DNA

Part:BBa_K3343001:Design

Designed by: Marijn van den Brink   Group: iGEM20_Leiden   (2020-09-08)


DNAzyme with peroxidase activity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An adenine residue was incorporated at the 3' end of the part to increases the activity of the DNAzyme and make it more tolerable for low pH.


Source

This part is not derived from a specific genomic sequence, but the human genome contains many potential G-quadruplex-forming sequences that possibly play important roles in some biological processes (General Peroxidase Activity of G-Quadruplex-Hemin Complexes and Its Application in Ligand Screening, Cheng et al., 2009).

References